Whole-cell patch-clamp recording of nicotinic acetylcholine receptors in adult Brugia malayi muscle
Identifieur interne : 002F73 ( Main/Exploration ); précédent : 002F72; suivant : 002F74Whole-cell patch-clamp recording of nicotinic acetylcholine receptors in adult Brugia malayi muscle
Auteurs : A. P. Robertson [États-Unis] ; S. K. Buxton [États-Unis] ; R. J. Martin [États-Unis]Source :
- Parasitology international [ 1383-5769 ] ; 2013.
Descripteurs français
- KwdFr :
- Acétylcholine (pharmacologie), Agonistes nicotiniques (pharmacologie), Animaux, Antihelminthiques (pharmacologie), Brugia malayi (), Brugia malayi (physiologie), Canaux ioniques (), Canaux ioniques (métabolisme), Cellules musculaires (), Cellules musculaires (physiologie), Composés de béphénium (pharmacologie), Femelle, Filariose lymphatique (parasitologie), Lévamisole (pharmacologie), Phénylènediamines (pharmacologie), Pyrantel (pharmacologie), Récepteurs nicotiniques (), Récepteurs nicotiniques (physiologie), Techniques de patch-clamp (), Électrophysiologie.
- MESH :
- métabolisme : Canaux ioniques.
- parasitologie : Filariose lymphatique.
- pharmacologie : Acétylcholine, Agonistes nicotiniques, Antihelminthiques, Composés de béphénium, Lévamisole, Phénylènediamines, Pyrantel.
- physiologie : Brugia malayi, Cellules musculaires, Récepteurs nicotiniques.
- Animaux, Brugia malayi, Canaux ioniques, Cellules musculaires, Femelle, Récepteurs nicotiniques, Techniques de patch-clamp, Électrophysiologie.
English descriptors
- KwdEn :
- Acetylcholine (pharmacology), Animals, Anthelmintics (pharmacology), Bephenium Compounds (pharmacology), Brugia malayi (drug effects), Brugia malayi (physiology), Electrophysiology, Elephantiasis, Filarial (parasitology), Female, Ion Channels (drug effects), Ion Channels (metabolism), Levamisole (pharmacology), Muscle Cells (drug effects), Muscle Cells (physiology), Nicotinic Agonists (pharmacology), Patch-Clamp Techniques (methods), Phenylenediamines (pharmacology), Pyrantel (pharmacology), Receptors, Nicotinic (drug effects), Receptors, Nicotinic (physiology).
- MESH :
- chemical , drug effects : Ion Channels, Receptors, Nicotinic.
- chemical , metabolism : Ion Channels.
- chemical , pharmacology : Acetylcholine, Anthelmintics, Bephenium Compounds, Levamisole, Nicotinic Agonists, Phenylenediamines, Pyrantel.
- drug effects : Brugia malayi, Muscle Cells.
- methods : Patch-Clamp Techniques.
- parasitology : Elephantiasis, Filarial.
- physiology : Brugia malayi, Muscle Cells, Receptors, Nicotinic.
- Animals, Electrophysiology, Female.
Abstract
Lymphatic filariasis is a debilitating disease caused by clade III parasites like
Url:
DOI: 10.1016/j.parint.2013.03.008
PubMed: 23562945
PubMed Central: 3744629
Affiliations:
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Le document en format XML
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P." last="Robertson">A. P. Robertson</name><affiliation wicri:level="4"><nlm:aff id="A1">Dept. Biomedical Science, College of Veterinary Medicine, Iowa State University, Ames</nlm:aff><orgName type="university">Université d'État de l'Iowa</orgName><country>États-Unis</country><placeName><settlement type="city">Ames (Iowa)</settlement><region type="state">Iowa</region></placeName></affiliation></author><author><name sortKey="Buxton, S K" sort="Buxton, S K" uniqKey="Buxton S" first="S. K." last="Buxton">S. K. Buxton</name><affiliation wicri:level="4"><nlm:aff id="A1">Dept. Biomedical Science, College of Veterinary Medicine, Iowa State University, Ames</nlm:aff><orgName type="university">Université d'État de l'Iowa</orgName><country>États-Unis</country><placeName><settlement type="city">Ames (Iowa)</settlement><region type="state">Iowa</region></placeName></affiliation></author><author><name sortKey="Martin, R J" sort="Martin, R J" uniqKey="Martin R" first="R. J." last="Martin">R. J. Martin</name><affiliation wicri:level="4"><nlm:aff id="A1">Dept. Biomedical Science, College of Veterinary Medicine, Iowa State University, Ames</nlm:aff><orgName type="university">Université d'État de l'Iowa</orgName><country>États-Unis</country><placeName><settlement type="city">Ames (Iowa)</settlement><region type="state">Iowa</region></placeName></affiliation></author></analytic><series><title level="j">Parasitology international</title><idno type="ISSN">1383-5769</idno><idno type="eISSN">1873-0329</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetylcholine (pharmacology)</term><term>Animals</term><term>Anthelmintics (pharmacology)</term><term>Bephenium Compounds (pharmacology)</term><term>Brugia malayi (drug effects)</term><term>Brugia malayi (physiology)</term><term>Electrophysiology</term><term>Elephantiasis, Filarial (parasitology)</term><term>Female</term><term>Ion Channels (drug effects)</term><term>Ion Channels (metabolism)</term><term>Levamisole (pharmacology)</term><term>Muscle Cells (drug effects)</term><term>Muscle Cells (physiology)</term><term>Nicotinic Agonists (pharmacology)</term><term>Patch-Clamp Techniques (methods)</term><term>Phenylenediamines (pharmacology)</term><term>Pyrantel (pharmacology)</term><term>Receptors, Nicotinic (drug effects)</term><term>Receptors, Nicotinic (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acétylcholine (pharmacologie)</term><term>Agonistes nicotiniques (pharmacologie)</term><term>Animaux</term><term>Antihelminthiques (pharmacologie)</term><term>Brugia malayi ()</term><term>Brugia malayi (physiologie)</term><term>Canaux ioniques ()</term><term>Canaux ioniques (métabolisme)</term><term>Cellules musculaires ()</term><term>Cellules musculaires (physiologie)</term><term>Composés de béphénium (pharmacologie)</term><term>Femelle</term><term>Filariose lymphatique (parasitologie)</term><term>Lévamisole (pharmacologie)</term><term>Phénylènediamines (pharmacologie)</term><term>Pyrantel (pharmacologie)</term><term>Récepteurs nicotiniques ()</term><term>Récepteurs nicotiniques (physiologie)</term><term>Techniques de patch-clamp ()</term><term>Électrophysiologie</term></keywords><keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>Ion Channels</term><term>Receptors, Nicotinic</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Ion Channels</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Acetylcholine</term><term>Anthelmintics</term><term>Bephenium Compounds</term><term>Levamisole</term><term>Nicotinic Agonists</term><term>Phenylenediamines</term><term>Pyrantel</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Brugia malayi</term><term>Muscle Cells</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Patch-Clamp Techniques</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Canaux ioniques</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Acétylcholine</term><term>Agonistes nicotiniques</term><term>Antihelminthiques</term><term>Composés de béphénium</term><term>Lévamisole</term><term>Phénylènediamines</term><term>Pyrantel</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Brugia malayi</term><term>Cellules musculaires</term><term>Récepteurs nicotiniques</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Brugia malayi</term><term>Muscle Cells</term><term>Receptors, Nicotinic</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Electrophysiology</term><term>Female</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Brugia malayi</term><term>Canaux ioniques</term><term>Cellules musculaires</term><term>Femelle</term><term>Récepteurs nicotiniques</term><term>Techniques de patch-clamp</term><term>Électrophysiologie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Lymphatic filariasis is a debilitating disease caused by clade III parasites like <italic>Brugia malayi</italic> and <italic>Wuchereria bancrofti</italic>. Current recommended treatment regimen for this disease relies on albendazole, ivermectin and diethylcarbamazine, none of which targets the nicotinic acetylcholine receptors in these parasitic nematodes. Our aim therefore has been to develop adult <italic>B. malayi</italic> for electrophysiological recordings to aid in characterizing the ion channels in this parasite as anthelmintic target sites. In that regard, we recently demonstrated the amenability of adult <italic>B. malayi</italic> to patch-clamp recordings and presented results on the single-channel properties of nAChR in this nematode. We have built on this by recording whole-cell nAChR currents from adult <italic>B. malayi</italic> muscle. Acetylcholine, levamisole, pyrantel, bephenium and tribendimidine activated the receptors on <italic>B. malayi</italic> muscle, producing robust currents ranging from > 200 pA to ~1.5 nA. Levamisole completely inhibited motility of the adult <italic>B. malayi</italic> within 10 min and after 60 min, motility had recovered back to control values.</p></div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Iowa</li></region><settlement><li>Ames (Iowa)</li></settlement><orgName><li>Université d'État de l'Iowa</li></orgName></list><tree><country name="États-Unis"><region name="Iowa"><name sortKey="Robertson, A P" sort="Robertson, A P" uniqKey="Robertson A" first="A. P." last="Robertson">A. P. Robertson</name></region><name sortKey="Buxton, S K" sort="Buxton, S K" uniqKey="Buxton S" first="S. K." last="Buxton">S. K. Buxton</name><name sortKey="Martin, R J" sort="Martin, R J" uniqKey="Martin R" first="R. J." last="Martin">R. J. Martin</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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